5 Simple Statements About attune flow cytometer Explained
5 Simple Statements About attune flow cytometer Explained
Blog Article
Information-enriched characterization of mobile populations. Morphological information and facts from pictures can include towards the richness of flow cytometry information. As an example, the determine demonstrates an otherwise conventional apoptosis assay using Annexin V and PI, introducing mobile imaging to characterize cells in Every populace to expose morphologically distinct options. These insights could not happen to be acquired from multiplex staining by itself.
As samples are obtained within the Attune CytPix Flow Cytometer, the significant-velocity brightfield digicam captures and shops photos of detected activities in a charge approximately six,000 photographs for every second, depending upon the flow charge and image dimensions. For increased versatility, Attune Cytometric Software package lets you change the graphic seize frequency as necessary and choose a selected gate for impression seize, if demanded.
(B) Uncooked 264.seven macrophages will often be annotated inaccurately with respect to particle count due to their complexity and noncircular borders. The number of singlets obtainable for analysis increases as soon as the consumer trains and employs a far more correct product.
Discover new insights into your experimental information by visualizing populations utilizing merged fluorescence and morphology details. Our software interface wizards help downsampling, concatenation (merged data files) and dimensionality applications which have been created into the program for an intuitive, streamlined knowledge Assessment expertise.
*Pricing is indicative only and is particularly topic to vary. You should contact your Agilent agent with any concerns. The information established forth herein just isn't a suggestion or possibly a dedication, both of that happen to be subject matter to credit acceptance, conditions and terms, and availability with Agilent. Agilent Fiscal Answers is out there in picked nations around the world.
Right here, a highly trained person has gated singlets confidently. Following analyzing the handbook singlet gate, the CytPix image-derived parameter ParticleCount reveals this gate incorporates much more than four% aggregates.
Optimizing gating system. Even robust handbook singlet gating is mistake-inclined and continues to be a subjective determination level in almost all flow cytometry assays. Imaging can be employed to substantiate and alter gates to incorporate only one cells of desire.
Our researchers will style panels for you personally as often times you will need—gratis, no products invest in essential.
The Attune NxT cytometer would be the reputable workhorse instrument your lab can depend upon for reliable and trustworthy functionality. The Attune CytPix model includes a higher-velocity brightfield digital camera and AI-pushed picture Assessment application, with latest updates like equipment Mastering tools and an intuitive consumer interface, enabling enhanced details precision, precision, and more quickly insights into sample biology.
Customers can view event illustrations or photos in serious-time to confirm sample top quality, optimize protocols, and history the morphology of every cell population identified for Assessment. Imaging seize also Gains from acoustic concentrating, which helps to position the cells in order that a pointy, centered image is obtained.
Seem waves empower specific cell alignment, leading to Excellent facts high-quality and ~10X speedier sample flow charges.
Software Take note: Automatic impression Investigation lessens person-to-consumer variability in flow cytometry gating approaches
Leverage the strength of Innovative technologies and innovation to speed up your discoveries. Belief within the Attune Flow Cytometer family members to deliver the dependability, speed, and precision that established your do the job aside.
Flexible payment strategy from Agilent Economic Solutions permit you to obtain the essential instrumentation you would like for speedy venture, or to improve your lab.
At the heart of each Attune Flow Cytometer lies our innovative acoustic focusing technological innovation, which utilizes sound waves to meticulously align cells throughout the flow cell. This revolutionary method surpasses traditional hydrodynamic approaches, leading to faster processing speeds, exceptional knowledge good quality, and elevated sample throughput.
Because the cell line had Formerly been read more analyzed within the Attune CytPix Flow Cytometer, the scientists went back again to the photographs and had been able to document the microbial infection at the very least five days earlier.
Agilent Benefit Promise guarantees you at the least a decade of instrument use with the date you buy. If not, We're going to credit score you While using the residual worth of the system towards an upgraded design.
What seemed to be a homogeneous population is definitely more various—an Perception that should be regarded when interpreting effects.